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85072401

Sigma

 

SP2/O-Ag14 Cell Line murine

Non-producing hybridoma

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Description

ApplicationThis cell line is a non-Ig-secreting or synthesising line derived from a cell line created by the fusion of BABL/c mouse spleen and the mouse myeloma P3X63Ag8. These cells are resistant to 8-azaguanine (20 μg/ml) and do not survive in media containing HAT.
Maintain cultures at 3-9 × 105 cells/ml. Inubate at 37 oC and 5% CO2 (HAT sensitive).
Recommended culture medium: RPMI-1640 or DMEM + 2 mM L-Glutamine + 10% FBS.

Properties

shipped indry ice
storage temp.−196°C

Safety

WGK Germany1